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fluorescent calcium indicator gcamp6f  (Addgene inc)


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    Addgene inc fluorescent calcium indicator gcamp6f
    Calcium imaging of responses to auditory stimuli. (a) Experimental setup. ( b) Example single plane of a zebrafish larval brain expressing nuclear localized <t>GCaMP6f</t> under the control of the HuC promoter. (c) Visualization of all neurons segmented from a single larva colored by depth. (d) Stimulus train. ( e) Average Δ F / F traces over all neurons ( n = 186,675) from all fish ( n = 12) for each block of the experiment. Note: (a) A larva mounted in low melting point agarose is illuminated by two perpendicular sheets of light. Acoustic stimuli are delivered by a speaker affixed to the back wall. Calcium activity is captured with a water‐immersion objective. (b) Image was averaged over the entire recording after motion correction. (c) Black is most ventral, white most dorsal. Example neurons are highlighted in red, with their respective calcium activity traces over time represented below. (d) Experimental tones of 531 Hz (pink) and 1189 Hz (purple) each act as the standard and deviant stimulus in different blocks. Control tones of 355 Hz, 794 Hz, and 1778 Hz enable each frequency to be presented at the same probability in the many standards control blocks. (e) Grey boxes indicate the timings of the stimulus blocks. Stimulus times for the experimental tones when not standard are illustrated above each graph.
    Fluorescent Calcium Indicator Gcamp6f, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fluorescent+calcium+indicator+gcamp6f/gcamp6s/pmc11946781-41-25-19
    Average 90 stars, based on 1 article reviews
    fluorescent calcium indicator gcamp6f - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Evidence for Auditory Stimulus‐Specific Adaptation But Not Deviance Detection in Larval Zebrafish Brains"

    Article Title: Evidence for Auditory Stimulus‐Specific Adaptation But Not Deviance Detection in Larval Zebrafish Brains

    Journal: The Journal of Comparative Neurology

    doi: 10.1002/cne.70046

    Calcium imaging of responses to auditory stimuli. (a) Experimental setup. ( b) Example single plane of a zebrafish larval brain expressing nuclear localized GCaMP6f under the control of the HuC promoter. (c) Visualization of all neurons segmented from a single larva colored by depth. (d) Stimulus train. ( e) Average Δ F / F traces over all neurons ( n = 186,675) from all fish ( n = 12) for each block of the experiment. Note: (a) A larva mounted in low melting point agarose is illuminated by two perpendicular sheets of light. Acoustic stimuli are delivered by a speaker affixed to the back wall. Calcium activity is captured with a water‐immersion objective. (b) Image was averaged over the entire recording after motion correction. (c) Black is most ventral, white most dorsal. Example neurons are highlighted in red, with their respective calcium activity traces over time represented below. (d) Experimental tones of 531 Hz (pink) and 1189 Hz (purple) each act as the standard and deviant stimulus in different blocks. Control tones of 355 Hz, 794 Hz, and 1778 Hz enable each frequency to be presented at the same probability in the many standards control blocks. (e) Grey boxes indicate the timings of the stimulus blocks. Stimulus times for the experimental tones when not standard are illustrated above each graph.
    Figure Legend Snippet: Calcium imaging of responses to auditory stimuli. (a) Experimental setup. ( b) Example single plane of a zebrafish larval brain expressing nuclear localized GCaMP6f under the control of the HuC promoter. (c) Visualization of all neurons segmented from a single larva colored by depth. (d) Stimulus train. ( e) Average Δ F / F traces over all neurons ( n = 186,675) from all fish ( n = 12) for each block of the experiment. Note: (a) A larva mounted in low melting point agarose is illuminated by two perpendicular sheets of light. Acoustic stimuli are delivered by a speaker affixed to the back wall. Calcium activity is captured with a water‐immersion objective. (b) Image was averaged over the entire recording after motion correction. (c) Black is most ventral, white most dorsal. Example neurons are highlighted in red, with their respective calcium activity traces over time represented below. (d) Experimental tones of 531 Hz (pink) and 1189 Hz (purple) each act as the standard and deviant stimulus in different blocks. Control tones of 355 Hz, 794 Hz, and 1778 Hz enable each frequency to be presented at the same probability in the many standards control blocks. (e) Grey boxes indicate the timings of the stimulus blocks. Stimulus times for the experimental tones when not standard are illustrated above each graph.

    Techniques Used: Imaging, Expressing, Control, Blocking Assay, Activity Assay

    Related Articles

    Injection:

    Article Title: TetrODrive: an open-source microdrive for combined electrophysiology and optophysiology.
    Article Snippet: .. Before inserting the microdrive, we injected 600 nl of an adeno-associated virus at a rate of 50 nl min−1 through a pulled glass pipette (Nanoliter 2000, World Precision Instruments), coding for the fluorescent calcium indicator GCaMP6f (AAV.Syn.Flex.GCaMP6f.WPRE.SV40, 1E13 particles/ml, Addgene) into the left VTA (anterior-posterior: −4.2 mm, medial-lateral: −0.5 mm, dorsal-ventral: 4.2 mm). ..

    Virus:

    Article Title: TetrODrive: an open-source microdrive for combined electrophysiology and optophysiology.
    Article Snippet: .. Before inserting the microdrive, we injected 600 nl of an adeno-associated virus at a rate of 50 nl min−1 through a pulled glass pipette (Nanoliter 2000, World Precision Instruments), coding for the fluorescent calcium indicator GCaMP6f (AAV.Syn.Flex.GCaMP6f.WPRE.SV40, 1E13 particles/ml, Addgene) into the left VTA (anterior-posterior: −4.2 mm, medial-lateral: −0.5 mm, dorsal-ventral: 4.2 mm). ..

    Transferring:

    Article Title: TetrODrive: an open-source microdrive for combined electrophysiology and optophysiology.
    Article Snippet: .. Before inserting the microdrive, we injected 600 nl of an adeno-associated virus at a rate of 50 nl min−1 through a pulled glass pipette (Nanoliter 2000, World Precision Instruments), coding for the fluorescent calcium indicator GCaMP6f (AAV.Syn.Flex.GCaMP6f.WPRE.SV40, 1E13 particles/ml, Addgene) into the left VTA (anterior-posterior: −4.2 mm, medial-lateral: −0.5 mm, dorsal-ventral: 4.2 mm). ..

    Bioprocessing:

    Article Title: TetrODrive: an open-source microdrive for combined electrophysiology and optophysiology.
    Article Snippet: .. Before inserting the microdrive, we injected 600 nl of an adeno-associated virus at a rate of 50 nl min−1 through a pulled glass pipette (Nanoliter 2000, World Precision Instruments), coding for the fluorescent calcium indicator GCaMP6f (AAV.Syn.Flex.GCaMP6f.WPRE.SV40, 1E13 particles/ml, Addgene) into the left VTA (anterior-posterior: −4.2 mm, medial-lateral: −0.5 mm, dorsal-ventral: 4.2 mm). ..

    Generated:

    Article Title: Evidence for Auditory Stimulus‐Specific Adaptation But Not Deviance Detection in Larval Zebrafish Brains
    Article Snippet: .. We generated larvae from a stock of adult transgenic zebrafish ( Danio rerio , Tg(elavl3:H2B‐GCaMP6f), ZFIN identifier: ZDB‐ALT‐150916‐4, RRID: Addgene_67159) with targeted expression of the fluorescent calcium indicator GCaMP6f in the nuclei of neurons (Chen et al. ), maintained on a TLN background at a density of 10–15 fish per liter. .. Fish were obtained from the Zebrafish International Resource Center and housed locally in a dedicated zebrafish facility at the University of Queensland.

    Transgenic Assay:

    Article Title: Evidence for Auditory Stimulus‐Specific Adaptation But Not Deviance Detection in Larval Zebrafish Brains
    Article Snippet: .. We generated larvae from a stock of adult transgenic zebrafish ( Danio rerio , Tg(elavl3:H2B‐GCaMP6f), ZFIN identifier: ZDB‐ALT‐150916‐4, RRID: Addgene_67159) with targeted expression of the fluorescent calcium indicator GCaMP6f in the nuclei of neurons (Chen et al. ), maintained on a TLN background at a density of 10–15 fish per liter. .. Fish were obtained from the Zebrafish International Resource Center and housed locally in a dedicated zebrafish facility at the University of Queensland.

    Expressing:

    Article Title: Evidence for Auditory Stimulus‐Specific Adaptation But Not Deviance Detection in Larval Zebrafish Brains
    Article Snippet: .. We generated larvae from a stock of adult transgenic zebrafish ( Danio rerio , Tg(elavl3:H2B‐GCaMP6f), ZFIN identifier: ZDB‐ALT‐150916‐4, RRID: Addgene_67159) with targeted expression of the fluorescent calcium indicator GCaMP6f in the nuclei of neurons (Chen et al. ), maintained on a TLN background at a density of 10–15 fish per liter. .. Fish were obtained from the Zebrafish International Resource Center and housed locally in a dedicated zebrafish facility at the University of Queensland.



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    fluorescent calcium indicator gcamp6f  (Addgene inc)
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    Addgene inc fluorescent calcium indicator gcamp6f
    Calcium imaging of responses to auditory stimuli. (a) Experimental setup. ( b) Example single plane of a zebrafish larval brain expressing nuclear localized <t>GCaMP6f</t> under the control of the HuC promoter. (c) Visualization of all neurons segmented from a single larva colored by depth. (d) Stimulus train. ( e) Average Δ F / F traces over all neurons ( n = 186,675) from all fish ( n = 12) for each block of the experiment. Note: (a) A larva mounted in low melting point agarose is illuminated by two perpendicular sheets of light. Acoustic stimuli are delivered by a speaker affixed to the back wall. Calcium activity is captured with a water‐immersion objective. (b) Image was averaged over the entire recording after motion correction. (c) Black is most ventral, white most dorsal. Example neurons are highlighted in red, with their respective calcium activity traces over time represented below. (d) Experimental tones of 531 Hz (pink) and 1189 Hz (purple) each act as the standard and deviant stimulus in different blocks. Control tones of 355 Hz, 794 Hz, and 1778 Hz enable each frequency to be presented at the same probability in the many standards control blocks. (e) Grey boxes indicate the timings of the stimulus blocks. Stimulus times for the experimental tones when not standard are illustrated above each graph.
    Fluorescent Calcium Indicator Gcamp6f, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fluorescent+calcium+indicator+gcamp6f/gcamp6s/pmc11946781-41-25-19
    Average 90 stars, based on 1 article reviews
    fluorescent calcium indicator gcamp6f - by Bioz Stars, 2026-09
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    transgenic mice expressing the fluorescence calcium indicator gcamp6f under the thy1 promoter gp5.17 mice  (Jackson Laboratory)
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    Calcium imaging of responses to auditory stimuli. (a) Experimental setup. ( b) Example single plane of a zebrafish larval brain expressing nuclear localized <t>GCaMP6f</t> under the control of the HuC promoter. (c) Visualization of all neurons segmented from a single larva colored by depth. (d) Stimulus train. ( e) Average Δ F / F traces over all neurons ( n = 186,675) from all fish ( n = 12) for each block of the experiment. Note: (a) A larva mounted in low melting point agarose is illuminated by two perpendicular sheets of light. Acoustic stimuli are delivered by a speaker affixed to the back wall. Calcium activity is captured with a water‐immersion objective. (b) Image was averaged over the entire recording after motion correction. (c) Black is most ventral, white most dorsal. Example neurons are highlighted in red, with their respective calcium activity traces over time represented below. (d) Experimental tones of 531 Hz (pink) and 1189 Hz (purple) each act as the standard and deviant stimulus in different blocks. Control tones of 355 Hz, 794 Hz, and 1778 Hz enable each frequency to be presented at the same probability in the many standards control blocks. (e) Grey boxes indicate the timings of the stimulus blocks. Stimulus times for the experimental tones when not standard are illustrated above each graph.
    Transgenic Mice Expressing The Fluorescence Calcium Indicator Gcamp6f Under The Thy1 Promoter Gp5.17 Mice, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fluorescent+calcium+indicator+gcamp6f/c57bl+6j+mice/pm37015224-201-8-21
    Average 90 stars, based on 1 article reviews
    transgenic mice expressing the fluorescence calcium indicator gcamp6f under the thy1 promoter gp5.17 mice - by Bioz Stars, 2026-09
    90/100 stars
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    Image Search Results


    Calcium imaging of responses to auditory stimuli. (a) Experimental setup. ( b) Example single plane of a zebrafish larval brain expressing nuclear localized GCaMP6f under the control of the HuC promoter. (c) Visualization of all neurons segmented from a single larva colored by depth. (d) Stimulus train. ( e) Average Δ F / F traces over all neurons ( n = 186,675) from all fish ( n = 12) for each block of the experiment. Note: (a) A larva mounted in low melting point agarose is illuminated by two perpendicular sheets of light. Acoustic stimuli are delivered by a speaker affixed to the back wall. Calcium activity is captured with a water‐immersion objective. (b) Image was averaged over the entire recording after motion correction. (c) Black is most ventral, white most dorsal. Example neurons are highlighted in red, with their respective calcium activity traces over time represented below. (d) Experimental tones of 531 Hz (pink) and 1189 Hz (purple) each act as the standard and deviant stimulus in different blocks. Control tones of 355 Hz, 794 Hz, and 1778 Hz enable each frequency to be presented at the same probability in the many standards control blocks. (e) Grey boxes indicate the timings of the stimulus blocks. Stimulus times for the experimental tones when not standard are illustrated above each graph.

    Journal: The Journal of Comparative Neurology

    Article Title: Evidence for Auditory Stimulus‐Specific Adaptation But Not Deviance Detection in Larval Zebrafish Brains

    doi: 10.1002/cne.70046

    Figure Lengend Snippet: Calcium imaging of responses to auditory stimuli. (a) Experimental setup. ( b) Example single plane of a zebrafish larval brain expressing nuclear localized GCaMP6f under the control of the HuC promoter. (c) Visualization of all neurons segmented from a single larva colored by depth. (d) Stimulus train. ( e) Average Δ F / F traces over all neurons ( n = 186,675) from all fish ( n = 12) for each block of the experiment. Note: (a) A larva mounted in low melting point agarose is illuminated by two perpendicular sheets of light. Acoustic stimuli are delivered by a speaker affixed to the back wall. Calcium activity is captured with a water‐immersion objective. (b) Image was averaged over the entire recording after motion correction. (c) Black is most ventral, white most dorsal. Example neurons are highlighted in red, with their respective calcium activity traces over time represented below. (d) Experimental tones of 531 Hz (pink) and 1189 Hz (purple) each act as the standard and deviant stimulus in different blocks. Control tones of 355 Hz, 794 Hz, and 1778 Hz enable each frequency to be presented at the same probability in the many standards control blocks. (e) Grey boxes indicate the timings of the stimulus blocks. Stimulus times for the experimental tones when not standard are illustrated above each graph.

    Article Snippet: We generated larvae from a stock of adult transgenic zebrafish ( Danio rerio , Tg(elavl3:H2B‐GCaMP6f), ZFIN identifier: ZDB‐ALT‐150916‐4, RRID: Addgene_67159) with targeted expression of the fluorescent calcium indicator GCaMP6f in the nuclei of neurons (Chen et al. ), maintained on a TLN background at a density of 10–15 fish per liter.

    Techniques: Imaging, Expressing, Control, Blocking Assay, Activity Assay